Composite

Part:BBa_K759010:Design

Designed by: Ami Kawahata   Group: iGEM12_HokkaidoU_Japan   (2012-09-20)


P3HB producing construct (without a promoter)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 614
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 225
    Illegal NgoMIV site found at 340
    Illegal NgoMIV site found at 574
    Illegal NgoMIV site found at 886
    Illegal NgoMIV site found at 1165
    Illegal NgoMIV site found at 1578
    Illegal NgoMIV site found at 1645
    Illegal AgeI site found at 313
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3583


Design Notes

Nothing to remark.


Source

phaA and phaB was cloned from pGEM phbCAB which carries the gene of Ralstonia Eutropha.

References